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tryple™ express enzyme cell dissociation reagent  (Thermo Fisher)


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    Thermo Fisher tryple™ express enzyme cell dissociation reagent
    Tryple™ Express Enzyme Cell Dissociation Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tryple+express+enzyme+reagent/bio_rxiv__2025__01__22__634029-154-12-18
    Average 90 stars, based on 1 article reviews
    tryple™ express enzyme cell dissociation reagent - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Virus:

    Article Title: N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma
    Article Snippet: cells were incubated with 5 μmol/L of Bodipy 581/591 C11 (ThermoFisher, cat. no. D3861) in complete medium for 30 minutes at 37°C. .. Cells were collected using TrypLE Express Enzyme reagent (Gibco cat. no. 126050), washed, and resuspended in ice-cold PBS. .. Data acquisition and analysis were performed in a BD FACSyphony A5 flow cytometer (BD Biosciences) using BD FACSDiva Software (RRID:SCR 00

    Article Title: Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages
    Article Snippet: from 40 embryos was pooled to generate Replicate 1 and tissue from 45 embryos was pooled to generate Replicate 2. .. Tissue was dissociated for 5 minutes at 37°C in TrypLE Express Enzyme reagent (Thermo Fisher 12604013), with shaking. .. The dissociation reagent was quenched with 10% FBS, and cells were subsequently washed in a 0.01% BSA/PBS solution.

    Article Title: A ribonucleoprotein octamer for targeted siRNA delivery.
    Article Snippet: viability was measured using the CellTiter-Blue (AlamarBlue) Cell Viability Assay kit (Promega), following the manufacturer’s protocol. .. To probe the cell cycle profiles, cells were first trypsinized with TrypLE Express Enzyme reagent (Thermo Fisher) and washed twice with DPBS. .. After incubation with Vybrant DyeCycle Orange Stain (Thermo Fisher) at 37 °C for 30 min, the stained cells were analysed on a flow cytometer

    Subcloning:

    Article Title: N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma
    Article Snippet: cells were incubated with 5 μmol/L of Bodipy 581/591 C11 (ThermoFisher, cat. no. D3861) in complete medium for 30 minutes at 37°C. .. Cells were collected using TrypLE Express Enzyme reagent (Gibco cat. no. 126050), washed, and resuspended in ice-cold PBS. .. Data acquisition and analysis were performed in a BD FACSyphony A5 flow cytometer (BD Biosciences) using BD FACSDiva Software (RRID:SCR 00

    Article Title: Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages
    Article Snippet: from 40 embryos was pooled to generate Replicate 1 and tissue from 45 embryos was pooled to generate Replicate 2. .. Tissue was dissociated for 5 minutes at 37°C in TrypLE Express Enzyme reagent (Thermo Fisher 12604013), with shaking. .. The dissociation reagent was quenched with 10% FBS, and cells were subsequently washed in a 0.01% BSA/PBS solution.

    Article Title: A ribonucleoprotein octamer for targeted siRNA delivery.
    Article Snippet: viability was measured using the CellTiter-Blue (AlamarBlue) Cell Viability Assay kit (Promega), following the manufacturer’s protocol. .. To probe the cell cycle profiles, cells were first trypsinized with TrypLE Express Enzyme reagent (Thermo Fisher) and washed twice with DPBS. .. After incubation with Vybrant DyeCycle Orange Stain (Thermo Fisher) at 37 °C for 30 min, the stained cells were analysed on a flow cytometer

    Recombinant:

    Article Title: N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma
    Article Snippet: cells were incubated with 5 μmol/L of Bodipy 581/591 C11 (ThermoFisher, cat. no. D3861) in complete medium for 30 minutes at 37°C. .. Cells were collected using TrypLE Express Enzyme reagent (Gibco cat. no. 126050), washed, and resuspended in ice-cold PBS. .. Data acquisition and analysis were performed in a BD FACSyphony A5 flow cytometer (BD Biosciences) using BD FACSDiva Software (RRID:SCR 00

    Article Title: Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages
    Article Snippet: from 40 embryos was pooled to generate Replicate 1 and tissue from 45 embryos was pooled to generate Replicate 2. .. Tissue was dissociated for 5 minutes at 37°C in TrypLE Express Enzyme reagent (Thermo Fisher 12604013), with shaking. .. The dissociation reagent was quenched with 10% FBS, and cells were subsequently washed in a 0.01% BSA/PBS solution.

    Article Title: A ribonucleoprotein octamer for targeted siRNA delivery.
    Article Snippet: viability was measured using the CellTiter-Blue (AlamarBlue) Cell Viability Assay kit (Promega), following the manufacturer’s protocol. .. To probe the cell cycle profiles, cells were first trypsinized with TrypLE Express Enzyme reagent (Thermo Fisher) and washed twice with DPBS. .. After incubation with Vybrant DyeCycle Orange Stain (Thermo Fisher) at 37 °C for 30 min, the stained cells were analysed on a flow cytometer

    Modification:

    Article Title: N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma
    Article Snippet: cells were incubated with 5 μmol/L of Bodipy 581/591 C11 (ThermoFisher, cat. no. D3861) in complete medium for 30 minutes at 37°C. .. Cells were collected using TrypLE Express Enzyme reagent (Gibco cat. no. 126050), washed, and resuspended in ice-cold PBS. .. Data acquisition and analysis were performed in a BD FACSyphony A5 flow cytometer (BD Biosciences) using BD FACSDiva Software (RRID:SCR 00

    Article Title: Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages
    Article Snippet: from 40 embryos was pooled to generate Replicate 1 and tissue from 45 embryos was pooled to generate Replicate 2. .. Tissue was dissociated for 5 minutes at 37°C in TrypLE Express Enzyme reagent (Thermo Fisher 12604013), with shaking. .. The dissociation reagent was quenched with 10% FBS, and cells were subsequently washed in a 0.01% BSA/PBS solution.

    Article Title: A ribonucleoprotein octamer for targeted siRNA delivery.
    Article Snippet: viability was measured using the CellTiter-Blue (AlamarBlue) Cell Viability Assay kit (Promega), following the manufacturer’s protocol. .. To probe the cell cycle profiles, cells were first trypsinized with TrypLE Express Enzyme reagent (Thermo Fisher) and washed twice with DPBS. .. After incubation with Vybrant DyeCycle Orange Stain (Thermo Fisher) at 37 °C for 30 min, the stained cells were analysed on a flow cytometer

    Lysis:

    Article Title: N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma
    Article Snippet: cells were incubated with 5 μmol/L of Bodipy 581/591 C11 (ThermoFisher, cat. no. D3861) in complete medium for 30 minutes at 37°C. .. Cells were collected using TrypLE Express Enzyme reagent (Gibco cat. no. 126050), washed, and resuspended in ice-cold PBS. .. Data acquisition and analysis were performed in a BD FACSyphony A5 flow cytometer (BD Biosciences) using BD FACSDiva Software (RRID:SCR 00

    Article Title: Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages
    Article Snippet: from 40 embryos was pooled to generate Replicate 1 and tissue from 45 embryos was pooled to generate Replicate 2. .. Tissue was dissociated for 5 minutes at 37°C in TrypLE Express Enzyme reagent (Thermo Fisher 12604013), with shaking. .. The dissociation reagent was quenched with 10% FBS, and cells were subsequently washed in a 0.01% BSA/PBS solution.

    Article Title: A ribonucleoprotein octamer for targeted siRNA delivery.
    Article Snippet: viability was measured using the CellTiter-Blue (AlamarBlue) Cell Viability Assay kit (Promega), following the manufacturer’s protocol. .. To probe the cell cycle profiles, cells were first trypsinized with TrypLE Express Enzyme reagent (Thermo Fisher) and washed twice with DPBS. .. After incubation with Vybrant DyeCycle Orange Stain (Thermo Fisher) at 37 °C for 30 min, the stained cells were analysed on a flow cytometer

    Western Blot:

    Article Title: N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma
    Article Snippet: cells were incubated with 5 μmol/L of Bodipy 581/591 C11 (ThermoFisher, cat. no. D3861) in complete medium for 30 minutes at 37°C. .. Cells were collected using TrypLE Express Enzyme reagent (Gibco cat. no. 126050), washed, and resuspended in ice-cold PBS. .. Data acquisition and analysis were performed in a BD FACSyphony A5 flow cytometer (BD Biosciences) using BD FACSDiva Software (RRID:SCR 00

    Article Title: Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages
    Article Snippet: from 40 embryos was pooled to generate Replicate 1 and tissue from 45 embryos was pooled to generate Replicate 2. .. Tissue was dissociated for 5 minutes at 37°C in TrypLE Express Enzyme reagent (Thermo Fisher 12604013), with shaking. .. The dissociation reagent was quenched with 10% FBS, and cells were subsequently washed in a 0.01% BSA/PBS solution.

    Article Title: A ribonucleoprotein octamer for targeted siRNA delivery.
    Article Snippet: viability was measured using the CellTiter-Blue (AlamarBlue) Cell Viability Assay kit (Promega), following the manufacturer’s protocol. .. To probe the cell cycle profiles, cells were first trypsinized with TrypLE Express Enzyme reagent (Thermo Fisher) and washed twice with DPBS. .. After incubation with Vybrant DyeCycle Orange Stain (Thermo Fisher) at 37 °C for 30 min, the stained cells were analysed on a flow cytometer

    Bicinchoninic Acid Protein Assay:

    Article Title: N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma
    Article Snippet: cells were incubated with 5 μmol/L of Bodipy 581/591 C11 (ThermoFisher, cat. no. D3861) in complete medium for 30 minutes at 37°C. .. Cells were collected using TrypLE Express Enzyme reagent (Gibco cat. no. 126050), washed, and resuspended in ice-cold PBS. .. Data acquisition and analysis were performed in a BD FACSyphony A5 flow cytometer (BD Biosciences) using BD FACSDiva Software (RRID:SCR 00

    Article Title: Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages
    Article Snippet: from 40 embryos was pooled to generate Replicate 1 and tissue from 45 embryos was pooled to generate Replicate 2. .. Tissue was dissociated for 5 minutes at 37°C in TrypLE Express Enzyme reagent (Thermo Fisher 12604013), with shaking. .. The dissociation reagent was quenched with 10% FBS, and cells were subsequently washed in a 0.01% BSA/PBS solution.

    Article Title: A ribonucleoprotein octamer for targeted siRNA delivery.
    Article Snippet: viability was measured using the CellTiter-Blue (AlamarBlue) Cell Viability Assay kit (Promega), following the manufacturer’s protocol. .. To probe the cell cycle profiles, cells were first trypsinized with TrypLE Express Enzyme reagent (Thermo Fisher) and washed twice with DPBS. .. After incubation with Vybrant DyeCycle Orange Stain (Thermo Fisher) at 37 °C for 30 min, the stained cells were analysed on a flow cytometer

    SYBR Green Assay:

    Article Title: N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma
    Article Snippet: cells were incubated with 5 μmol/L of Bodipy 581/591 C11 (ThermoFisher, cat. no. D3861) in complete medium for 30 minutes at 37°C. .. Cells were collected using TrypLE Express Enzyme reagent (Gibco cat. no. 126050), washed, and resuspended in ice-cold PBS. .. Data acquisition and analysis were performed in a BD FACSyphony A5 flow cytometer (BD Biosciences) using BD FACSDiva Software (RRID:SCR 00

    Article Title: Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages
    Article Snippet: from 40 embryos was pooled to generate Replicate 1 and tissue from 45 embryos was pooled to generate Replicate 2. .. Tissue was dissociated for 5 minutes at 37°C in TrypLE Express Enzyme reagent (Thermo Fisher 12604013), with shaking. .. The dissociation reagent was quenched with 10% FBS, and cells were subsequently washed in a 0.01% BSA/PBS solution.

    Article Title: A ribonucleoprotein octamer for targeted siRNA delivery.
    Article Snippet: viability was measured using the CellTiter-Blue (AlamarBlue) Cell Viability Assay kit (Promega), following the manufacturer’s protocol. .. To probe the cell cycle profiles, cells were first trypsinized with TrypLE Express Enzyme reagent (Thermo Fisher) and washed twice with DPBS. .. After incubation with Vybrant DyeCycle Orange Stain (Thermo Fisher) at 37 °C for 30 min, the stained cells were analysed on a flow cytometer

    Reporter Assay:

    Article Title: N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma
    Article Snippet: cells were incubated with 5 μmol/L of Bodipy 581/591 C11 (ThermoFisher, cat. no. D3861) in complete medium for 30 minutes at 37°C. .. Cells were collected using TrypLE Express Enzyme reagent (Gibco cat. no. 126050), washed, and resuspended in ice-cold PBS. .. Data acquisition and analysis were performed in a BD FACSyphony A5 flow cytometer (BD Biosciences) using BD FACSDiva Software (RRID:SCR 00

    Article Title: Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages
    Article Snippet: from 40 embryos was pooled to generate Replicate 1 and tissue from 45 embryos was pooled to generate Replicate 2. .. Tissue was dissociated for 5 minutes at 37°C in TrypLE Express Enzyme reagent (Thermo Fisher 12604013), with shaking. .. The dissociation reagent was quenched with 10% FBS, and cells were subsequently washed in a 0.01% BSA/PBS solution.

    Article Title: A ribonucleoprotein octamer for targeted siRNA delivery.
    Article Snippet: viability was measured using the CellTiter-Blue (AlamarBlue) Cell Viability Assay kit (Promega), following the manufacturer’s protocol. .. To probe the cell cycle profiles, cells were first trypsinized with TrypLE Express Enzyme reagent (Thermo Fisher) and washed twice with DPBS. .. After incubation with Vybrant DyeCycle Orange Stain (Thermo Fisher) at 37 °C for 30 min, the stained cells were analysed on a flow cytometer

    Control:

    Article Title: N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma
    Article Snippet: cells were incubated with 5 μmol/L of Bodipy 581/591 C11 (ThermoFisher, cat. no. D3861) in complete medium for 30 minutes at 37°C. .. Cells were collected using TrypLE Express Enzyme reagent (Gibco cat. no. 126050), washed, and resuspended in ice-cold PBS. .. Data acquisition and analysis were performed in a BD FACSyphony A5 flow cytometer (BD Biosciences) using BD FACSDiva Software (RRID:SCR 00

    Article Title: Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages
    Article Snippet: from 40 embryos was pooled to generate Replicate 1 and tissue from 45 embryos was pooled to generate Replicate 2. .. Tissue was dissociated for 5 minutes at 37°C in TrypLE Express Enzyme reagent (Thermo Fisher 12604013), with shaking. .. The dissociation reagent was quenched with 10% FBS, and cells were subsequently washed in a 0.01% BSA/PBS solution.

    Article Title: A ribonucleoprotein octamer for targeted siRNA delivery.
    Article Snippet: viability was measured using the CellTiter-Blue (AlamarBlue) Cell Viability Assay kit (Promega), following the manufacturer’s protocol. .. To probe the cell cycle profiles, cells were first trypsinized with TrypLE Express Enzyme reagent (Thermo Fisher) and washed twice with DPBS. .. After incubation with Vybrant DyeCycle Orange Stain (Thermo Fisher) at 37 °C for 30 min, the stained cells were analysed on a flow cytometer

    Transgenic Assay:

    Article Title: N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma
    Article Snippet: cells were incubated with 5 μmol/L of Bodipy 581/591 C11 (ThermoFisher, cat. no. D3861) in complete medium for 30 minutes at 37°C. .. Cells were collected using TrypLE Express Enzyme reagent (Gibco cat. no. 126050), washed, and resuspended in ice-cold PBS. .. Data acquisition and analysis were performed in a BD FACSyphony A5 flow cytometer (BD Biosciences) using BD FACSDiva Software (RRID:SCR 00

    Article Title: Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages
    Article Snippet: from 40 embryos was pooled to generate Replicate 1 and tissue from 45 embryos was pooled to generate Replicate 2. .. Tissue was dissociated for 5 minutes at 37°C in TrypLE Express Enzyme reagent (Thermo Fisher 12604013), with shaking. .. The dissociation reagent was quenched with 10% FBS, and cells were subsequently washed in a 0.01% BSA/PBS solution.

    Article Title: A ribonucleoprotein octamer for targeted siRNA delivery.
    Article Snippet: viability was measured using the CellTiter-Blue (AlamarBlue) Cell Viability Assay kit (Promega), following the manufacturer’s protocol. .. To probe the cell cycle profiles, cells were first trypsinized with TrypLE Express Enzyme reagent (Thermo Fisher) and washed twice with DPBS. .. After incubation with Vybrant DyeCycle Orange Stain (Thermo Fisher) at 37 °C for 30 min, the stained cells were analysed on a flow cytometer

    Software:

    Article Title: N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma
    Article Snippet: cells were incubated with 5 μmol/L of Bodipy 581/591 C11 (ThermoFisher, cat. no. D3861) in complete medium for 30 minutes at 37°C. .. Cells were collected using TrypLE Express Enzyme reagent (Gibco cat. no. 126050), washed, and resuspended in ice-cold PBS. .. Data acquisition and analysis were performed in a BD FACSyphony A5 flow cytometer (BD Biosciences) using BD FACSDiva Software (RRID:SCR 00

    Article Title: Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages
    Article Snippet: from 40 embryos was pooled to generate Replicate 1 and tissue from 45 embryos was pooled to generate Replicate 2. .. Tissue was dissociated for 5 minutes at 37°C in TrypLE Express Enzyme reagent (Thermo Fisher 12604013), with shaking. .. The dissociation reagent was quenched with 10% FBS, and cells were subsequently washed in a 0.01% BSA/PBS solution.

    Article Title: A ribonucleoprotein octamer for targeted siRNA delivery.
    Article Snippet: viability was measured using the CellTiter-Blue (AlamarBlue) Cell Viability Assay kit (Promega), following the manufacturer’s protocol. .. To probe the cell cycle profiles, cells were first trypsinized with TrypLE Express Enzyme reagent (Thermo Fisher) and washed twice with DPBS. .. After incubation with Vybrant DyeCycle Orange Stain (Thermo Fisher) at 37 °C for 30 min, the stained cells were analysed on a flow cytometer

    Staining:

    Article Title: N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma
    Article Snippet: cells were incubated with 5 μmol/L of Bodipy 581/591 C11 (ThermoFisher, cat. no. D3861) in complete medium for 30 minutes at 37°C. .. Cells were collected using TrypLE Express Enzyme reagent (Gibco cat. no. 126050), washed, and resuspended in ice-cold PBS. .. Data acquisition and analysis were performed in a BD FACSyphony A5 flow cytometer (BD Biosciences) using BD FACSDiva Software (RRID:SCR 00

    Article Title: Hedgehog signaling activates a mammalian heterochronic gene regulatory network controlling differentiation timing across lineages
    Article Snippet: from 40 embryos was pooled to generate Replicate 1 and tissue from 45 embryos was pooled to generate Replicate 2. .. Tissue was dissociated for 5 minutes at 37°C in TrypLE Express Enzyme reagent (Thermo Fisher 12604013), with shaking. .. The dissociation reagent was quenched with 10% FBS, and cells were subsequently washed in a 0.01% BSA/PBS solution.

    Article Title: A ribonucleoprotein octamer for targeted siRNA delivery.
    Article Snippet: viability was measured using the CellTiter-Blue (AlamarBlue) Cell Viability Assay kit (Promega), following the manufacturer’s protocol. .. To probe the cell cycle profiles, cells were first trypsinized with TrypLE Express Enzyme reagent (Thermo Fisher) and washed twice with DPBS. .. After incubation with Vybrant DyeCycle Orange Stain (Thermo Fisher) at 37 °C for 30 min, the stained cells were analysed on a flow cytometer



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